Frecuencia y patotipos de Escherichia coli diarrogénica en niños peruanos con y sin diarrea

• DOI: 10.17843/rpmesp.2011.281.450
• Publication Date: 2018-08-06T15:20:45Z
• Authors: Theresa J. Ochoa; Erik H. Mercado; David Durand; Fulton P. Rivera; Susan Mosquito; Carmen Contreras; Maribel Riveros; Angela Lluque; Francesca Barletta; Ana Prada; Joaquim Ruiz
• Keywords: enterotoxigenic Escherichia coli; 0301 basic medicine; Multiplex polymerase chain reaction; diffusely adherent Escherichia coli; FOS: Health sciences; Gene; Perú; Enteropathogenic Escherichia coli; Endocrinology; Outbreak of Cronobacter sakazakii Infections; genetic variability; Peru; 11. Sustainability; Child; Pathogenesis and Virulence of Escherichia coli; Internal medicine; Escherichia coli Infections; 0303 health sciences; Virulence; Life Sciences; molecular mechanics; Polymerase chain reaction; 3. Good health; Infectious Diseases; Niño; Medicine; diarrheagenic Escherichia coli; Escherichia coli, Diarrhea, Child, Peru, Virulence; Escherichia coli, Diarrea, Niño, Perú, Virulencia; Diarrhea; https://purl.org/pe-repo/ocde/ford#3.03.05; Shiga toxin producing Escherichia coli; prevalence; enteropathogenic Escherichia coli infection; enteroaggregative Escherichia coli; gene frequency; Microbiology; 03 medical and health sciences; Biochemistry, Genetics and Molecular Biology; Health Sciences; Escherichia coli; Genetics; Humans; controlled study; Biology; Virulencia; enteroinvasive Escherichia coli; Pathogen; bacterial virulence; non age distribution; pathotype; Infant; Gastrointestinal Viral Infections and Vaccines Development; bacterial strain; FOS: Biological sciences; Diarrea

Introduction. Diarrheagenic E. coli (DEC) are a major cause of diarrhea in children in developing countries. However, they are not part of routine diagnosis in clinical laboratories. Objectives. To determine the Dec prevalence in Peruvian children and to describe the genetic variability of these strains. Materials and methods. A total of 8 003 E. coli strains previously isolated from eight different studies of diarrhea in children, mainly from peri-urban areas of Lima, were analyzed. Diagnosis of Dec was done with Multiplex real-time PCR using genes for each of the 6 Dec groups. Conventional PCR was performed for the detection of additional virulence genes. Results. Globally, the mean prevalence in diarrhea samples (n=4,243) was: enteroaggregative E. coli (EAEC) 9.9%, enteropathogenic E. coli (EPEC) 8.5%, enterotoxigenic E. coli (ETEC) 6.9%, diffusely adherent E. coli (DAEC) 4.8%, Shiga toxin-producing E. coli (STEC) 0.8% and enteroinvasive E. coli (EIEC) 0.6%. The relative frequency of each pathogen varies according to the age and type of study. The main pathotypes in control samples (n=3,760) were EPEC (10.9%) and EAEC (10.4%). An important variability in the virulence genes frequency and molecular resistance mechanisms for each pathotype was found, without differences between diarrhea and control groups. Conclusions. Dec are a major cause of diarrhea in Peruvian children. These pathogens are highly heterogeneous. Additional studies are required to determine the prevalence in rural areas of Peru and in severe diarrhea cases.<br/>Introduction. Diarrheagenic E. coli (DEC) are a major causa of diarrhea in children in developing countries. However, they are not part of routine diagnosis in clinical laboratories. Objectives. À déterminer la prévalence du DEC chez les enfants péruviens et à décrire la variabilité génétique de ces couches. Materials and methods. A total of 8 003 E. coli strains previously isolated from eight different studies of diarrhea in children, mainly from peri-urban areas of Lima, were analyszed. Diagnostic des DEC was done with Multiplex Real-time PCR using genes for each of the 6 DEC groups. Conventional PCR was performed for the detection of additional virulence genes. Résultats Globalement, la prévalence moyenne dans les échantillons de diarrhée (n=4,243) was : entérogroupe E. coli (EAEC) 9,9 %, entéropathogène E. coli (EPEC) 8,5 %, entérotoxinogène E. coli (ETEC) 6,9 %, diffusion adhérente E. coli (DAEC) 4,8 %, Shiga toxin-producing E. coli (STEC) 0,8 % et entérinvasive E. coli (EIEC) 0,6 %. The relative frequency of each pathogen varies according to the age and the type of study. The main pathotypes in control samples (n= 3,760) were EPEC (10,9 %) and EAEC (10,4 %). An important variability in the virulence genes frequency and molecular resistance mechanisms for each pathotype was found, without differences between diarrhea and control groups. Conclusions. DEC are a major causa of diarrhea in Peruvian children. These pathogens are highly heterogeneous. Des études supplémentaires sont nécessaires pour déterminer la prévalence dans les zones rurales du Pérou et dans les cas de diarrhée sévère.<br/>مقدمة. تعد الإشريكية القولونية المسببة للإسهال (DEC) سببًا رئيسيًا للإسهال لدى الأطفال في البلدان النامية. ومع ذلك، فهي ليست جزءًا من التشخيص الروتيني في المختبرات السريرية. الأهداف. لتحديد مدى انتشار هذه السلالة في أطفال بيرو ووصف التباين الجيني لهذه السلالات. المواد والأساليب - تم تحليل ما مجموعه 8003 من سلالات الإشريكية القولونية المعزولة سابقًا من ثماني دراسات مختلفة للإسهال لدى الأطفال، خاصة من المناطق شبه الحضرية في ليما. تم تشخيص DEC باستخدام تفاعل البوليميراز المتسلسل المتعدد في الوقت الفعلي باستخدام الجينات لكل مجموعة من مجموعات DEC 6. تم إجراء تفاعل البوليميراز المتسلسل التقليدي للكشف عن جينات الفوعة الإضافية. النتائج. على الصعيد العالمي، كان متوسط الانتشار في عينات الإسهال (العدد= 4،243) هو: الإشريكية القولونية المعوية (EAEC) 9.9 ٪، والإشريكية القولونية المعوية (EPEC) 8.5 ٪، والإشريكية القولونية المعوية (ETEC) 6.9 ٪، والإشريكية القولونية المنتشرة (DAEC) 4.8 ٪، والإشريكية القولونية المنتجة للسموم (STEC) 0.8 ٪ والإشريكية القولونية المعوية (EIEC) 0.6 ٪. يختلف التكرار النسبي لكل عامل ممرض وفقًا لعمر ونوع الدراسة. كانت الأنماط المرضية الرئيسية في عينات التحكم (العدد=3,760) هي EPEC (10.9 ٪) و EAEC (10.4 ٪). تم العثور على تباين مهم في تردد جينات الفوعة وآليات المقاومة الجزيئية لكل نمط مرضي، دون اختلافات بين الإسهال ومجموعات التحكم. الاستنتاجات. DEC هي سبب رئيسي للإسهال لدى الأطفال البيروفيين. هذه مسببات الأمراض غير متجانسة للغاية. ويلزم إجراء دراسات إضافية لتحديد مدى الانتشار في المناطق الريفية في بيرو وفي حالات الإسهال الشديد.<br/>Introduction. Diarrheagenic E. coli (DEC) are a major cause of diarrhea in children in developing countries. However, they are not part of routine diagnosis in clinical laboratories. Objectives. To determine the DEC prevalence in Peruvian children and to describe the genetic variability of these strains. Materials and methods. A total of 8 003 E. coli strains previously isolated from eight different studies of diarrhea in children, mainly from peri-urban areas of Lima, were analyzed. Diagnosis of DEC was done with Multiplex real-time PCR using genes for each of the 6 DEC groups. Conventional PCR was performed for the detection of additional virulence genes. Results. Globally, the mean prevalence in diarrhea samples (n=4,243) was: enteroaggregative E. coli (EAEC) 9.9%, enteropathogenic E. coli (EPEC) 8.5%, enterotoxigenic E. coli (ETEC) 6.9%, diffusely adherent E. coli (DAEC) 4.8%, Shiga toxin-producing E. coli (STEC) 0.8% and enteroinvasive E. coli (EIEC) 0.6%. The relative frequency of each pathogen varies according to the age and the type of study. The main pathotypes in control samples (n=3,760) were EPEC (10.9%) and EAEC (10.4%). An important variability in the virulence genes frequency and molecular resistance mechanisms for each pathotype was found, without differences between diarrhea and control groups. Conclusions. DEC are a major cause of diarrhea in Peruvian children. These pathogens are highly heterogeneous. Additional studies are required to determine the prevalence in rural areas of Peru and in severe diarrhea cases.<br/>