CaMKK2-Mediated Effects on Mechanically Induced Bone Formation in Male and Female Mice

• DOI: 10.18060/24525
• Publication Date: 2020-12-15T18:17:48Z
• Authors: Margaret Bello; Adam Warrick; Brett Mattingly; Justin Williams; Uma Sankar
• Keywords: Bone Formation

Background and Hypothesis: Ca2+/calmo-dulin-dependent protein kinase 2 (CaMKK2) is a serine-threonine that plays significant role in both anabolic catabolic pathways of bone remodeling. Mechanical loading translates an external force into biochemical structural changes. It has been shown deletion or inhibition CaMKK2 results increased density male female mice. We hypothesize the lack cells will result loading-induced mass accrual with no difference between Experimental Design Project Methods: The right tibia anesthetized 16-week-old wild-type (WT) knockout (KO) mice were loaded at Hz for 220 cycles peak forces specific to sex genotype. Loading was accomplished using electro actuator (Bose ElectroForce 3200; EnduraTEC, Minnetonka, MN, USA). This repeated 3, 5, 8 10 days after initial loading. non-loaded left served as internal control. Calcein alizarin red administered intraperitoneally on 9 16, respectively metabolically label newly formed bone. Nineteen loading, sacrificed. Blood long bones lower limbs collected analysis. Results: Using microcomputer tomography; dynamic histomorphometry; histology, immunohistochemistry, enzyme-linked immunosorbent assay real-time reverse transcription-polymerase chain reaction, we assess volume, formation rate, underlying mechanisms cellular molecular level. These data are forthcoming. Conclusion Potential Impact: With expanded knowledge how growth augmented, clinical outcomes related osteoporosis fracture healing, example, may be improved. through novel therapy these increases decreases healing time.