Proteomic profiling of HaCaT keratinocytes exposed to skin damaging detergents

• DOI: 10.18097/pbmc20176305405
• Publication Date: 2017-10-27T06:52:10Z
• Authors: A.L. Rusanov; N.A. Petushkova; E.V. Poverennaya; K.V. Nakhod; O.V. Larina; A.V. Lisitsa; N.G. Luzgina
• Keywords: HaCaT; Sodium dodecyl sulfate; Shotgun proteomics

The effects of sodium dodecyl sulfate (25 mg/ml) and Triton X-100 (12.5 mg/ml 25 on the HaCaT immortalized keratinocytes exposed to these surfactants for 48 h were studied. Using shotgun proteomics, a comparative analysis proteomic profiles control experimental cells after exposure was carried out. 260 common proteins identified in cells; 33 found all three treatments, but not cells. These apparently reflect nonspecific (universal) response toxic damage by surfactants. are associated with activation cell proliferation, changes functional activity their ER mitochondria, increased mRNA stability protein degradation processes possibility using as parameter cytotoxic is discussed. mass spectrometry proteomics data ("raw", "mgf" "xml" files) have been deposited ProteomeXchange Consortium via PRIDE partner repository dataset identifiers PXD007789 PXD007776.Issledovano vliianie dodetsilsul'fata natriia (v kontsentratsii mkg/ml) i tritona Kh-100 kontsentratsiiakh 12,5 na kletochnuiu kul'turu immortalizovannykh keratinotsitov linii v techenie ch. Metodom panoramnoĭ mass-spektrometrii proveden sravnitel'nyĭ analiz proteomnogo profilia kontrol'nykh kletok posle vozdeĭstviia poverkhnostno-aktivnymi veshchestvami (PAV). Vyiavleno belkov obshchikh dlia opytnykh belka, poiavliaiushchikhsia ne vstrechaiushchikhsia kontrole, chto, po-vidimomu, mozhet otrazhat' nespetsificheskiĭ (universal'nyĭ) otvet toksicheskoe povrezhdenie PAV. Dannye belki assotsiirovany s aktivatsieĭ protsessov proliferatsii kletok, izmeneniem funktsional'noĭ aktivnosti ikh ÉR mitokhondriĭ, povysheniem stabil'nosti mRNK degradatsii belka kletkakh. Obsuzhdaetsia vozmozhnost' ispol'zovaniia étikh kachestve nespetsificheskogo parametra otveta tsitotoksicheskoe povrezhdenie. Mass-spektrometricheskie dannye formatakh “raw” “mgf”) rezul'taty poiska peptidov/belkov (“xml” format) dostupny prosmotra publichnom depozitarii (identifikatory PXD007776).