Protective effect of LIF-huMSCs on the retina of diabetic model rats

• DOI: 10.18240/ijo.2021.10.06
• Publication Date: 2021-09-24T03:02:21Z
• Authors: Shan-Na Chen; Ying-Xue Ma; ; Song Chen; ; Guang-Hui He; ; Mei Han; ; Xiang Gao; ; Jun-Hua Wang; ; Bin Wu; ; Jian Wang
• Keywords: Electroretinography

To investigate the protective effect of human umbilical cord mesenchymal stem cells (hUCMSCs) modified by LIF gene on retinal function diabetic model rats and preliminarily explore possible mechanism.A stably transfected cell line hUCMSCs overexpressing leukemia inhibitory factor (LIF) was constructed. Overexpression verified fluorescent quantitative polymerase chain reaction (qPCR). Forty-eight adult Sprague-Dawley were randomly divided into a normal control group (group A), streptozotocin-induced B), at 3mo injected with empty vector-transfected C) or LIF-hUCMSCs D). Four weeks after intravitreal injection, analyses in all groups included using flash electroretinogram (F-ERG), blood vessel examination flat mounts perfused fluorescein isothiocyanate-dextran (FITC-dextran), structure sections hematoxylin eosin staining. Expression levels adiponectin (APN), high-sensitivity C-reactive protein (hs-CRP), neurotrophin-4 (NT-4) each detected immunohistochemistry, PCR, Western blotting, ELISA, respectively.A stable transgenic F-ERG FITC-dextran examinations revealed no abnormalities A, severe damage vessels B, improved C especially D. qPCR, blot progressively higher APN NT-4 expression C, D than A. hs-CRP significantly B D, (P<0.05).LIF-hUCMSCs protect retina upregulating downregulating retina.