IFNα2b/5-FU inhibits proliferation and cell cycle of squamous carcinoma cell line Cal27

• DOI: 10.18240/ijo.2025.04.01
• Publication Date: 2025-03-21T07:55:13Z
• Authors: Zong-Bo Wu; Bei Wang; ; Xi Peng; ; Jia-Song Wang; ; Ming-Chang Zhang; ; Wen Geng; ; Hua-Tao Xie
• Keywords: Thymidine phosphorylase; Terminal deoxynucleotidyl transferase

AIM: To investigate the pathological features of ocular surface squamous neoplasia (OSSN) and evaluate synergistic therapeutic effects interferon-α2b (IFNα2b) 5-fluorouracil (5-FU) on cellular proliferation, migration, apoptosis, cell cycle human oral carcinoma line Cal27. METHODS: Tissue specimens from OSSN were processed with hematoxylin-eosin (HE) immunofluorescence (IF) staining to characterize changes. We analyzed expression levels four pivotal proteins involved in 5-FU metabolism: interferon alpha receptor (IFNAR), thymidylate synthase (TS), thymidine phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD). Cal27 lines treated a spectrum concentrations IFNα2b 5-FU, either isolation or combination. Then, activity was measured utilizing CCK-8 assay dose-effect curves calculated, while tumor migration detected by scratch experiments. cells added non-constant ratio drug combination design corresponding index (CI) fraction affected (Fa) calculated CompuSyn software. Western blot conducted quantify TP, TS, DPD. Cell apoptosis flow cytometry terminal deoxynucleotidyl transferase-mediated dUTP nick labeling (TUNEL) assay. RESULTS: Treatment both inhibited proliferation. Except for lowest highest doses CI values all other groups below 1, suggesting interaction. Low diminished relative mobility cells, instead, stronger inhibitory effect observed when two drugs co-applied. The TP DPD dose-dependently increased at low concentration IFNα2b. Low-dose combined significantly proliferation G0/G1 phase compared monotherapy. Medium high could induce concentration-dependent manner. susceptibility treatment rates elevated CONCLUSION: Both administered individually combination, effectively suppress arrest cycle. increase antitumor potentially through up-regulating demonstrating between 5-FU.