Pathways of α-methylstyrene Oxidation by P. aeruginosa DS-26

• DOI: 10.18321/ectj241
• Publication Date: 2015-11-21T09:47:35Z
• Authors: D. B. Jussupova; L. A. Golovleva; A. A. Zhubanova; N. Sh. Akimbekov
• Keywords: Hydroxylation; Acetophenone

Today in world practice the biotechnological methods of cleaning environment from oil and products are widely used. They based on use microorganisms-destructors that able to utilize<br />hydrocarbons. The microbial object research was bacterial strain <em>P. aeruginosa</em> DS-26. This culture isolated sewage water rubber industry. grow a medium, which contained <em>α</em>-methylstyrene. compound, is used as an active component many industries, has a<br />strong toxic effect living organisms including humans. Different types metabolism play important role hydrocarbon degradation process Metabolism <em>α</em>-methylstyrene by DS-26 characterized variety depth carried out transformations. analysis obtained data allowed offering two ways oxidation α-methylstyrene. first way, primary, accompanied formation direct hydroxylation aromatic ring - cis-2.3-dihydroxy-1-isopropenyl-6-cyclohexene 3-isopropyl-catehol later metabolized meta-pathway with keto-acids. next, secondary pathway came true through α-phenylpropionic acid 4-methylbenzene alcohol acetophenone final product.<br />There researches devoted study biochemical conversion hydrocarbons. In order utilize specific compounds only one source carbon, microorganisms have actively maintain system enzymes. Investigation DS-<br />26 enzymes revealed oxidize results studying can be confirmation proposed above scheme primary α-methylstyrene metabolic oxidized