Assessment of the effects of cellular therapy on reproduction of the conditioned passive avoidance reflex in rats with quinoline-induced model of Huntington’s disease

• DOI: 10.18454/acen.2017.2.5
• Publication Date: 2025-01-31T21:04:01Z
• Authors: Alla V. Stavrovskaya; Ekaterina V. Novo...; Nina G. Yamshchikova; Artem S. Olshansky; Anastasiya S. Gus...; Eugeniya V. Konov...; Igor' A. Grivennikov; Sergey N. Illario...
• Keywords: 0303 health sciences; induced pluripotent stem cells; conditioned passive avoidance reflex; conditioned passive avoidance reflex, trace memory disturbance; neural progenitors; Neurosciences. Biological psychiatry. Neuropsychiatry; quinolinic acid; 3. Good health; 03 medical and health sciences; huntington’s disease; neurotransplantation; uinolinic acid; RC321-571; Huntington’s disease

Introduction. The model involving injection of quinolinic acid (QA) into the rat striatum simulates many clinical and morphological characteristics Huntingtons disease (HD). Searching for effective treatment methods is rather topical because fatality HD. One such to create a neuroprotective environment slow down current degenerative process and/or replace dead neurons. In particular, this can be performed by transplantation cells capable undergoing neuronal differentiation integration proper structural functional brain networks. Objective. To assess effectiveness safety neural progenitors differentiated from induced pluripotent stem (iPSCs) harvested healthy donor with QA-induced Materials methods. effects neurotransplantation on reproduction conditioned passive avoidance reflex were studied in rats HD QA caudate nuclei striatum. study group (n=8), human (1106 per 10 l normal saline unilaterally, injured side) derived iPSCs injected as transplanted material; was control group. responses tested using ShutАvoid 1.8.03 software Harvard apparatus (Panlab, Spain). Results. When testing responses, we found that reliably reduced responses. Neurotransplantation had clear therapeutic effect reinforced reflex. During entire period (7 days after exposure pain stimulus), experimental animals either did not visit dark compartment at all or visited it long latency period. Conclusions. Experimental iPSC derivatives allowance improve storage trace memory HD, which contributes correction cognitive impairments caused administration neurotoxin.