The Association of Human Leucocyte Antigen (HLA) Class I and II Genes with Cutaneous and Visceral Leishmaniasis in Iranian Patients: A Preliminary Case-Control Study

• DOI: 10.18502/ijpa.v18i2.13181
• Publication Date: 2023-07-17T08:07:44Z
• Authors: Mitra Eimanzadeh; Mehdi Mohebali; Morteza Zarrabi; Abbas Rahimi Foro...; Mohammad Kazemi; Homa Hajjaran; Zabih Zarei; Zahra Kakooei; Behnaz Akhoundi
• Keywords: Direct agglutination test

Background: Leishmaniasis is currently considered a re-emerging or emerging infection based on the geographic region. The outcome of leishmaniasis vastly depends Leishmania-host interaction. This preliminary study aimed to show association human leukocyte antigen (HLA) class I and II genes with healed non-healed cutaneous (CL), symptomatic asymptomatic visceral (VL) compared control groups in Iran. Methods: Ninety-five people, including 31 patients versus 64 individuals group, were enrolled. Among them, 20 had confirmed CL amastigote observation, 10 improved CL. Eleven suffering from VL direct agglutination test (Five six cases). Besides, they residents an endemic area northwest To select it was ensured that no history leishmaniasis. Peripheral blood samples collected each patient. After DNA extraction, HLA typing conducted using polymerase chain reaction - sequence-specific priming (PCR-SSP). Subsequently, data statistically analyzed by SPSS. Results: There statistical relationship between presence HLA-A26 CL, existence B38 allele, C1 allele VL, as well B1.4 (P˂0.05). Conclusion: primary finding indicates several have potential role susceptibility Iranian people VL.