Therapeutic Effects of Edaravone on Azoospermia: Free Radical Scavenging and Autophagy Modulation in Testicular Tissue of Mice

• DOI: 10.18502/jri.v23i2.8990
• Publication Date: 2022-03-16T08:42:52Z
• Authors: Mahsa Ghaffari Novin; Mohammadamin Sabb...; Mohammadreza Mafi...; Abbas Aliaghaei; Azar Afshar; Fakhroddin Aghaja...; Reza Soltani; Hamid Nazarian; Maryam Salimi; Ahad Hasan Seyed ...; Shabnam Abdi; Mohammad-Amin Abd...; Pourya Raee
• Keywords: Edaravone

Background: Chemotherapeutic agents such as cyclophosphamide and busulfan have been shown to a negative impact on the spermatogenesis process. Based this fact, objective of study was investigate effects edaravone in busulfan-induced mice. Methods: Forty adult male mice were equally divided into four groups: 1) control, 2) edaravone, 3) busulfan, 4) + edaravone. Then, sperm parameters, histopathological examinations, serum levels testosterone, follicle-stimulating hormone (FSH), luteinizing (LH) also assessed. Caspase-3, Beclin-1, ATG-7 mRNA determined using real-time PCR. Results: Our results revealed that treatment with azoospermia significantly improves including total count, morphology, viability (p<0.05). Furthermore, administration led significant increase testosterone (p<0.0001) FSH (p<0.001) levels, well testis weight (p<0.05) volume (p<0.01). Edaravone prevented decrease number testicular cells spermatogonia (p<0.0001), primary spermatocytes (p<0.001), round spermatids Sertoli (p<0.01), Leydig busulfan-treated Additionally, azoospermia, reduced percentage immature chromatin (p<0.0001). Following reactive oxygen species (ROS) an glutathione (GSH) production noted compared caspase-3 (p<0.05), genes expression decreased groups azoospermia. Conclusion: According our findings, can improve through free radical scavenging autophagy modulation tissue.