Cytarabine determination from urine for Toxicokinetic and Excretion studies by High- Performance Liquid Chromatography-Tandem Mass Spectrometry

• DOI: 10.18596/jotcsa.1190202
• Publication Date: 2023-05-30T18:03:19Z
• Authors: İbrahim DANİŞ; Ayşegül GÖLCÜ; Durişehvar ÜNAL
• Keywords: 03 medical and health sciences; 0302 clinical medicine; Analitik Kimya; Cytarabine;therapeutic drug monitoring;toxicokinetics;mass spectrometry; Analytical Chemistry

Cytarabine (Cyt) (also known as cytosine arabinoside (ara-C)) used in the treatment of acute myeloid leukemia (AML), lymphocytic (ALL). CYT applied high doses for can cause renal failure. Monitoring excreted urine drug levels help kidney For this reason, a method was developed and validated by HPLC-MSMS analysis, which is not included literature. In study, liquid chromatography (HPLC) with triple quadrupole Mass Spectrometric (MS/MS) determination Cyt from toxicokinetic evaluation. Positive MRM mode selected quantification Cyt. The product major fragment ion 244.0 > 112.0 m/z, IS 198.0 152.0 m/z. optimal MS parameters are follows Fragmentor 80 V, 70 Collision energy, 6, 9 respectively. A novel simple, high-throughput highly sensitive HPLC-MS/MS successfully urine. has simple one-step extraction short run time (2.0 minutes) analysis. proposed could be practical reliable excretion studies well Therapeutic Drug study humans without an invasive route