Long non-coding RNA RHPN1-AS1 promotes tumorigenesis and metastasis of ovarian cancer by acting as a ceRNA against miR-596 and upregulating LETM1

• DOI: 10.18632/aging.102911
• Publication Date: 2020-03-12T18:37:46Z
• Authors: Junrong Wang; Weimin Ding; Yingke Xu; Enfu Tao; Miaojun Mo; Wei Xu; Xu Cai; Xiaomin Chen; Junhui Yuan; Xiuying Wu
• Keywords: Competing Endogenous RNA; MALAT1

Background: In recent decades, long non-coding RNAs (lncRNAs) have been reported as crucial functional regulators involved in ovarian cancer.In the present study, we explored how lncRNA RHPN1-AS1 influences progression of epithelial cancer (EOC) through tumor cell-dependent mechanisms.Results: The expression EOC tissues was higher than that para-cancerous control tissues.High closely associated with poor prognosis patients.N6methyladenosine (m6A) improved stability methylation transcript by reducing RNA degradation, which resulted upregulation EOC.In vitro and vivo experiments showed promoted cell proliferation metastasis.RHPN1-AS1 acted a ceRNA to sponge miR-596, consequently increasing LETM1 activating FAK/PI3K/Akt signaling pathway.Conclusion: RHPN1-AS1-miR-596-LETM1 axis plays role progression.Our findings may provide promising drug targets for treatment.Methods: We determined aberrantly expressed lncRNAs via microarray analysis validated qRT-PCR.The examined dual-luciferase reporter assay RIP assay.The mechanism investigated gain-and loss-offunction studies both vitro.