NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma

• DOI: 10.18632/aging.103909
• Publication Date: 2020-11-18T05:05:27Z
• Authors: Wen-Jie Zhou; Hao-Yu Wang; Jie Zhang; Hai-Ying Dai; Zhi-Xian Yao; Zhong Zheng; Sun Meng-Yan; Ke Wu
• Keywords: 0301 basic medicine; 0303 health sciences; Epithelial-Mesenchymal Transition; Skin Neoplasms; Smad2 Protein; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Databases, Genetic; Humans; Neoplasm Invasiveness; RNA, Long Noncoding; Melanoma; Research Paper; Cell Proliferation; Signal Transduction

Melanoma is a skin malignancy with high mutation frequency of genetic alterations. MicroRNA (miR)-200b-3p involved in various cancers, while melanoma its bio-function remains unknown. In this study, we found that miR-200b-3p was down-regulated tissues and cell lines compared to benign nevus cells. Overexpression significantly inhibited the proliferation invasion According bioinformatics analysis sequencing data, supposed SMAD family member 2 (SMAD2) target gene nuclear enriched abundant transcript 1 (NEAT1) upstream long non-coding RNA (lncRNA) miR-200b-3p. These predictions were verified by western blotting quantitative real-time reverse transcription PCR (RT-qPCR). Luciferase reporter assays revealed NEAT1 up-regulated SMAD2 directly sponging vitro vivo, demonstrated both could promote cells, these effects reversed up-regulating addition, NEAT1/miR-200b-3p/SMAD2 axis promoted progression activating EMT signaling pathway immune responses. Taken together, promotes via activation EMT, related responses, which provides new insights into molecular mechanisms therapeutic targets for melanoma.