Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients
Gold standard (test)
Coronavirus
Cut-off
2019-20 coronavirus outbreak
DOI:
10.18632/aging.104020
Publication Date:
2020-11-01T23:56:13Z
AUTHORS (9)
ABSTRACT
The worldwide severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak has led to the rapid spread of disease (COVID-19). quantitative real time PCR (qPCR) is widely used as gold standard for clinical detection SARS-CoV-2. However, more and infected patients are relapsing after discharge, which suggests qPCR may fail detect virus in some cases. In this study, we selected 74 samples from 43 recovering inpatients Droplet Digital (ddPCR) synchronous blind detection, established a cutoff value ddPCR diagnosis COVID-19. results showed that at 0.04 copies/μL, sensitivity specificity 97.6% 100%, respectively. addition, also analyzed 18 retained 9 discharged who relapsed. Although all be negative, 12 positive, there was only one patient with two negative samples; other eight had least positive sample. These indicate could significantly improve accuracy COVID-19 diagnosis, especially low viral load, help reduce misdiagnosis during recovery.
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