Diagnostic and prognostic values of AKR1C3 and AKR1D1 in hepatocellular carcinoma

• DOI: 10.18632/aging.202380
• Publication Date: 2021-01-22T15:24:48Z
• Authors: Pengfei Zhu; Ruo Feng; Xu Lu; Yuan Liao; Zhicheng Du; Wenlong Zhai; Kunlun Chen
• Keywords: Male; 0301 basic medicine; 0303 health sciences; Carcinoma, Hepatocellular; Liver Neoplasms; Aldo-Keto Reductase Family 1 Member C3; Down-Regulation; Hep G2 Cells; Middle Aged; Prognosis; 3. Good health; 03 medical and health sciences; Gene Ontology; Area Under Curve; Case-Control Studies; Cell Line, Tumor; Gene Knockdown Techniques; Humans; Female; Protein Interaction Maps; RNA, Messenger; Oxidoreductases; Research Paper; Cell Proliferation; Proportional Hazards Models

Hepatocellular carcinoma (HCC) is the most common histological type of primary liver cancer and majority patients are diagnosed at an advanced stage have a poor prognosis. AKR1C3 (Aldo-keto reductase family 1 member C3) AKR1D1 D1) catalyze conversion aldehydes ketones to alcohols play crucial roles in multiple cancers. However, functions HCC remain unclear. In our study, data from public databases were selected as training validation sets, then 76 center chosen test set. Bioinformatics methods suggested was overexpressed down-regulated. The receiver operating characteristic curve (ROC) analysis performed area under (AUC) values above 0.7 (0.948, 0.836, respectively). Also, high expression low predicted prognosis short median survival time. Then, knockdown overexpression cells achieved with lentivirus. And both decreased cell proliferation, restrained viability, inhibited tumorigenesis. Moreover, gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses conducted results showed that might participate MAPK/ERK androgen receptor (AR) signaling pathway. Furthermore, AR phosphorylated ERK1/2 significantly reduced after suppression or AKR1D1. Collectively, serve candidate diagnostic prognostic biomarkers for provide potential targets treatment.