Long non-coding RNAs ENST00000429730.1 and MSTRG.93125.4 are associated with metabolic activity in tuberculosis lesions of sputum-negative tuberculosis patients

• DOI: 10.18632/aging.202634
• Publication Date: 2021-03-04T12:20:11Z
• Authors: Lin Wang; Zilu Wen; Hui Ma; Liwei Wu; Hui Chen; Yijun Zhu; Liangfei Niu; Qihang Wu; Hongwei Li; Lei Shi; Leilei Li; Leiyi Wan; Jun Wang; Ka-Wing Wong; Yanzheng Song
• Keywords: Adult; Male; 0301 basic medicine; 3. Good health; Young Adult; 03 medical and health sciences; Positron Emission Tomography Computed Tomography; Humans; Female; RNA, Long Noncoding; Tuberculosis, Pulmonary; Biomarkers; Research Paper

Accurate diagnosis of complete inactivation tuberculosis lesions is still a challenge with respect to sputum-negative tuberculosis. RNA-sequencing was conducted uncover potential lncRNA indicators metabolic activity in lesions. Lung tissues high and low demonstrated by fluorine-18-fluorodeoxyglucose positron emission tomography/computed tomography were collected from five patients for RNA-sequencing. Differentially-expressed mRNAs lncRNAs identified. Their correlations evaluated construct lncRNA-mRNA co-expression network, which degrees confirmed quantitative real-time PCR using samples 11 patients. Prediction efficiencies assessed receiver operating characteristic curve analysis. Bioinformatics analysis performed lncRNAs. 386 44 identified be differentially expressed. network significantly associated fibrillar collagen, platelet-derived growth factor binding, leukocyte migration involved inflammatory response. Seven (C1QB, CD68, CCL5, CCL19, MMP7, HLA-DMB, CYBB) two (ENST00000429730.1 MSTRG.93125.4) validated up-regulated. The area under the ENST00000429730.1 MSTRG.93125.4 0.750 0.813, respectively. Two might considered as