Matairesinol exerts anti-inflammatory and antioxidant effects in sepsis-mediated brain injury by repressing the MAPK and NF-κB pathways through up-regulating AMPK

• DOI: 10.18632/aging.203649
• Publication Date: 2021-10-27T18:47:07Z
• Authors: Qin Wu; Yuhua Wang; Qingfang Li
• Keywords: 0301 basic medicine; Brain Diseases; MAP Kinase Signaling System; Anti-Inflammatory Agents; NF-kappa B; Brain; AMP-Activated Protein Kinases; Antioxidants; Lignans; Cell Line; Rats; Up-Regulation; 3. Good health; Disease Models, Animal; 03 medical and health sciences; Sepsis; Animals; Mitogen-Activated Protein Kinases; Furans; Research Paper

Brain injury is a familiar complication of severe sepsis, in which excessive inflammation and oxidative stress are the main mechanisms leading to acute brain injury. Here, we focus on probing function mechanism Matairesinol (Mat) sepsis-mediated We established rat sepsis model by cecal ligation perforation (CLP) constructed an vitro treating neurons microglia with lipopolysaccharide (LPS). Rats cells were treated varying concentrations Mat, changes neural function, neuronal apoptosis, microglial activation, neuroinflammation expression factors tissues examined. Additionally, activation MAPK, NF-κB AMPK pathways was evaluated Western blot (WB) and/or immunohistochemistry (IHC). Our findings illustrated that Mat improved apoptosis weakened CLP rats. Meanwhile, hampered pro-inflammatory (TNF-α, IL-1β, IL-6, IFN-γ, IL-8, MCP1) facilitated contents glutathione peroxidase (GSH-Px) superoxide dismutase (SOD) microglia. Mechanistically, concentration-dependently dampened phosphorylation JNK rats LPS-stimulated up-regulated Nrf2 HO-1. Besides, expression. Compound C, specific inhibitor pathway, substantially reduced protection anti-inflammatory effects mediated Mat. Overall, exerts anti-oxidative up-regulating AMPK, thereby ameliorating