Identification and validation of anoikis-associated gene SNCG as a prognostic biomarker in gastric cancer

• DOI: 10.18632/aging.204626
• Publication Date: 2023-03-30T21:36:46Z
• Authors: Yi Li; Qin Pan; Mingxia Cheng; Zhengyuan Wu
• Keywords: Identification; Anoikis

Anoikis is a newly discovered form of apoptosis that was originally identified in the extracellular matrix (ECM). Recent studies have reported anoikis central to cancer metastasis. Here, SNCG as hub anoikis-associated gene GC and associated with prognosis patients GC. To screen genes connected GC, database Cancer Genome Atlas (TCGA) employed. For further validating these genes, Gene Expression Omnibus (GEO) dataset applied, Western blotting quantitative Real-Time PCR were carried out. identify we conducted analyses univariate Cox regression, differential expression, weighted co-expression network analysis (WGCNA). According constructed model prognosis. Following complex analysis, finally Indeed, K-M receiver operating characteristic suggested expression patterns can be used prognostic factors for survival. The survival trends verified validation cohort vitro experimental analyses. immune cell infiltration showed infiltrated cells varied among SNCG. Furthermore, due significant association risk signature patient age survival, this predict We suggest served Meanwhile, may potential overall