Long non-coding RNA MEG3 acts as a suppressor in breast cancer by regulating miR-330/CNN1

• DOI: 10.18632/aging.205419
• Publication Date: 2024-01-17T22:48:12Z
• Authors: Dandan Yi; Zetian Wang; Haojie Yang; Ru Wang; Xianbiao Shi; Zhijian Liu; Fazhan Xu; Qing Lu; Xiao Chu; Jianfeng Sang
• Keywords: MEG3

Background: The current study aimed to investigate the molecular mechanism of long non-coding RNA (lncRNA) MEG3 in development breast cancer. Methods: regulating relationships among lncRNA MEG3, miRNA-330 and CNN1 were predicted by bioinformatics analysis cancer samples Cancer Genome Atlas database. differential expression was first validated tissues cells. effects on malignant properties evaluated manipulating its MCF-7 BT-474 Rescue experiments, dual-luciferase assays, immunoprecipitation (RIP) experiments further used validate CNN1. Results: Bioinformatics showed that MEGs significantly downregulated tissues, while miR-330 upregulated. These expressions our cohort samples. High levels as well low associated with favorable overall survival. Overexpression inhibited cell viability, migration invasion, decreased cells S stage promoted apoptosis. Dual-luciferase reporter gene assay RIP could directly bind miR-330. Moreover, mimics basis overexpression ameliorated tumor-suppressing decreasing expression. Conclusion: Our indicated is a suppressor miR-330/CNN1 axis.