SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression

• DOI: 10.18632/oncotarget.13167
• Publication Date: 2016-11-07T20:13:46Z
• Authors: Tao Huang; Yan Ji; Dan Hu; Baozheng Chen; Hejun Zhang; Chao Li; Gang Chen; Xingguang Luo; Xiong-wei Zheng; Xiandong Lin
• Keywords: Adult; Male; Epstein-Barr Virus Infections; Herpesvirus 4, Human; 0303 health sciences; Sequence Analysis, RNA; Gene Expression Profiling; Computational Biology; Middle Aged; Cell Transformation, Viral; Real-Time Polymerase Chain Reaction; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Databases, Genetic; Host-Pathogen Interactions; Humans; Female; Gene Regulatory Networks; RNA, Long Noncoding; RNA, Messenger; RNA, Neoplasm; Research Paper; Aged

The Epstein-Barr virus (EBV) is associated with a variety of cancers, including gastric cancer, which has one the highest mortality rates all human cancers. Long non-coding RNAs (lncRNAs) have been suggested to important causal roles in cancer. However, interaction between lncRNAs and EBV not yet studied. To this end, we sequenced 11,311 144,826 protein-coding transcripts from four types tissue: non-EBV-infected carcinoma (EBVnGC) its adjacent normal tissue, EBV-associated (EBVaGC) tissue. Five showed EBVaGC-specific expression; those, (SNHG8) was validated using real-time PCR an independent cohort 88 paired cancer tissue samples. explore functions SNHG8, identified mRNA targets on lncRNA-mRNA co-expression network Illumina Body Map, contains RNA sequencing data mRNAs 16 tissues. SNHG8 lncRNA found affect several cancer-specific pathways target genes EBV. Our results reveal intertwined tumorigenesis mechanisms identify as highly possible candidate biomarker drug