Characterization and phylogenetic analysis of Krüppel-like transcription factor (KLF) gene family in tree shrews (Tupaia belangeri chinensis)
0303 health sciences
Binding Sites
Sequence Homology, Amino Acid
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Blotting, Western
Kruppel-Like Transcription Factors
Tupaiidae
Zinc Fingers
Sequence Analysis, DNA
Cell Line
Mice
03 medical and health sciences
Multigene Family
MCF-7 Cells
NIH 3T3 Cells
Animals
Humans
Amino Acid Sequence
Protein Processing, Post-Translational
Phylogeny
Research Paper
Cell Proliferation
DOI:
10.18632/oncotarget.13883
Publication Date:
2016-12-10T20:24:35Z
AUTHORS (9)
ABSTRACT
Krüppel-like factors (KLFs) are a family of zinc finger transcription factors regulating embryonic development and diseases. The phylogenetics of KLFs has not been studied in tree shrews, an animal lineage with a closer relationship to primates than rodents. Here, we identified 17 KLFs from Chinese tree shrew (Tupaia belangeri chinensis). KLF proteins are highly conserved among humans, monkeys, rats, mice and tree shrews compared to zebrafish and chickens. The CtBP binding site, Sin3A binding site and nuclear localization signals are largely conserved between tree shrews and human beings. Tupaia belangeri (Tb) KLF5 contains several conserved post-transcriptional modification motifs. Moreover, the mRNA and protein expression patterns of multiple tbKLFs are tissue-specific . TbKLF5, like hKLF5, significantly promotes NIH3T3 cell proliferation in vitro. These results provide insight for future studies regarding the structure and function of the tbKLF gene family.
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CITATIONS (18)
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