Characterization of tumor-associated B-cell subsets in patients with colorectal cancer

• DOI: 10.18632/oncotarget.1701
• Publication Date: 2015-09-15T23:39:30Z
• Authors: Alexander Shimabu...; Hans A. Schlößer; Luise Gryschok; Joke Malcher; Kerstin Wennhold; Maria Garcia-Marquez; Till Herbold; Laura S. Neuhaus; Hans J. Becker; Anne Fiedler; Pascal Scherwitz; Thomas Koslowsky; Roland Hake; Dirk L. Stippel; Arnulf H. Hölscher; Sebastian Eidt; Michael Hallek; Sebastian Theurich; Michael S. von Be...
• Keywords: Regulatory B cells; Circulating tumor cell

$('.header-date').hide();$('#titleAuthors').hide(); $('#abstractHeader').hide(); Alexander Shimabukuro-Vornhagen 1 , 2 * Hans A. Schlößer 3 Luise Gryschok Joke Malcher Kerstin Wennhold Maria Garcia-Marquez Till Herbold Laura S. Neuhaus J. Becker Anne Fiedler Pascal Scherwitz 4 Thomas Koslowsky 5 Roland Hake 6 Dirk L. Stippel Arnulf H. Hölscher Sebastian Eidt Michael Hallek Theurich von Bergwelt-Baildon Cologne Interventional Immunology, University of Cologne, Germany. Department I Internal Medicine, General, Visceral and Cancer Surgery, Marien Hospital, Brühl, St. Elisabeth Institute Pathology, This authors contributed equally to this work. Correspondence to: Bergwelt-Baildon, e-mail: [e-mail] Received: December 14, 2013      Accepted: April 28, 2014      Published: May 09, 2014 ABSTRACT Purpose: A precise understanding the mechanisms by which human immune cell subsets affect tumor biology will be critical for successful treatment cancer using immunotherapeutic approaches. Recent evidence suggests that B cells can both promote inhibit development progression tumors. The aim study was characterize composition B-cell infiltrates in colorectal cancers (CRC) order gain further insight into role CRC. Experimental Design: In we characterized primary tumors (n=38), metastases (n=6) blood (n=46) 51 patients with a diagnosis CRC 10 healthy controls. were analyzed flow cytometry or immunohistochemistry. Results: Peripheral contained higher percentage memory than age-matched Furthermore, within peripheral while typically devoid tumor-infiltrating cells. Tumor-associated enriched activated terminally differentiated Relevant proportions regulatory could only detected advanced metastases. Conclusion: constitute significant proportion infiltrate is an accumulation plasma suggestive specific response against tumor. However are also infiltrated considerable number