Assessment of antimicrobial and wound healing effects of Brevinin-2Ta against the bacteriumKlebsiella pneumoniaein dermally-wounded rats

• DOI: 10.18632/oncotarget.22797
• Publication Date: 2017-12-01T13:59:56Z
• Authors: Siqin Liu; Qilin Long; Yang Xu; Jun Wang; Zhongwei Xu; Lei Wang; Mei Zhou; Yuxin Wu; Tianbao Chen; Chris Shaw
• Keywords: 0301 basic medicine; angiogenesis; Klebsiella pneumoniae; 570; 03 medical and health sciences; inflammation; 610; brevinine; AMP; brevinine; inflammation; angiogenesis; Klebsiella pneumoniae; AMP; Research Paper; 3. Good health

Antimicrobial peptides (AMPs) are regarded as promising alternatives for antibiotics due to their inherent capacity prevent microbial drug resistance. Amphibians rich source of bioactive molecules, which provide numerous AMPs with various structures candidates. Here, we isolated and identified a novel AMP Brevinin-2Ta (B-2Ta) from the skin secretion European frog, Pelophylax kl. esculentus. In vitro studies revealed that it showed broad antimicrobial activities against S. aureus, E. coli C. albicans low cytotoxicity erythrocytes. Furthermore, examined anti-inflammation effect in vivo by using Klebsiella pneumoniae-infected Sprague-Dawley (SD) rats. The wound closure outcomes B-2Ta effectively restrained bacterial infection at dose 10 times minimal inhibitory concentration (MIC) during 14 days healing process. Ultra-structure analyses caused structural damage microorganism, culture found number microbes was significantly reduced end treatment. Immunohistochemistry inflammatory marker IL-10 endothelial cell CD31 suggested positive effects on status epithelial migration angiogenesis following treatment infected granulation tissues B-2Ta. These results exhibited continuous phase inflammation reduction acceleration B-2Ta-modulated re-epithelialisation K. pneumoniae Taken together, these data demonstrated has great potential be developed antibacterial agents clinic.