CIP4 promotes metastasis in triple-negative breast cancer and is associated with poor patient prognosis

• DOI: 10.18632/oncotarget.3351
• Publication Date: 2015-09-15T07:00:46Z
• Authors: Otto L.D. Cerqueira; Peter Truesdell; Tomas Baldassarre; Santiago A. Vilel...; Kathleen Watt; Jalna Meens; Harish Chander; Cynthia A.B. Osório; Fernando A. Soares; Eduardo M. Reis; Andrew W.B. Craig
• Keywords: Triple-negative breast cancer

// Otto L.D. Cerqueira 1 , Peter Truesdell 2 Tomas Baldassarre Santiago A. Vilella-Arias Kathleen Watt Jalna Meens Harish Chander Cynthia A.B. Osório 3 Fernando Soares 3, 4 Eduardo M. Reis 1, Andrew W.B. Craig Departamento de Bioquímica, Instituto Química, Universidade São Paulo, SP, Brazil Department of Biomedical and Molecular Sciences, Queen's University, Division Cancer Biology & Genetics, Research Institute, Kingston, ON, Canada Anatomic Pathology, A.C. Camargo Hospital, Nacional Ciência e Tecnologia em Oncogenômica, Correspondence to: W. Craig, e-mail: [e-mail] Reis, [e-mail] Keywords: metastasis, triple-negative breast cancer, CIP4 Received: October 22, 2014 Accepted: February 10, 2015 Published: March 19, ABSTRACT Signaling via epidermal growth factor receptor (EGFR) Src kinase pathways promote cancer (TNBC) cell invasion tumor metastasis. Here, we address the role Cdc42-interacting protein-4 (CIP4) in TNBC metastasis vivo profile expression human patients. In cells, knock-down (KD) led to less sustained activation Erk impaired motility compared control cells. This correlated with significant defects 3D surrounding extracellular matrix by KD cells when grown as spheroid colonies. mammary orthotopic xenograft assays using both (MDA-MB-231, HCC 1806) rat MTLn3 silencing had no overt effect on growth, but significantly reduced incidence lung metastases each model. invasive cancers, high levels was associated stage, HER2 subtypes, risk progression metastatic disease. Together, these results implicate promoting TNBCs.