Activation of homologous recombination DNA repair in human skin fibroblasts continuously exposed to X-ray radiation

• DOI: 10.18632/oncotarget.4946
• Publication Date: 2015-09-29T17:43:03Z
• Authors: Andreyan N. Osipov; Anna Grekhova; Margarita Pustova...; Ivan V. Ozerov; Petr Eremin; Natalia Vorobyeva; Natalia Lazareva; Andrey Pulin; Alex Zhavoronkov; Sergey Roumiantsev; Dmitry Klokov; Ilya Eremin
• Keywords: Non-homologous end joining

// Andreyan N. Osipov 1, 2, 3, 4 , Anna Grekhova 5 Margarita Pustovalova 2 Ivan V. Ozerov 1 Petr Eremin Natalia Vorobyeva 3 Lazareva Andrey Pulin Alex Zhavoronkov 4, 6, 7 Sergey Roumiantsev 8 Dmitry Klokov 9 Ilya State Research Center - Burnasyan Federal Medical Biophysical of Biological Agency (SRC-FMBC), Moscow 123098, Russia Semenov Institute Chemical Physics, Russian Academy Sciences, 119991, Rogachev Pediatric Hematology, Oncology and Immunology, 117997, Physics Technology, Dolgoprudny, Region 141700, Emanuel for Biochemical 6 Insilico Medicine, Inc, ETC, Johns Hopkins University, Baltimore, MD 21218, USA The Biogerontology Foundation, BGRF, London W1J 5NE, UK N.I. Pirogov National Canadian Nuclear Laboratories, Chalk River, ON K0J1P0, Canada Correspondence to: Osipov, e-mail: [e-mail] Keywords: DNA DSB repair, homologous recombination, human fibroblasts, X-rays, continuous irradiation Received: April 08, 2015 Accepted: July 31, Published: August 13, ABSTRACT Molecular cellular responses to protracted ionizing radiation exposures are poorly understood. Using immunofluorescence microscopy, we studied the kinetics repair foci formation in normal fibroblasts exposed X-rays at a dose rate 4.5 mGy/min up h. We showed that both number γH2AX their integral fluorescence intensity grew linearly with time A plateau was observed between h exposure, indicating state balance double-strand breaks. In contrast, formed by recombination protein RAD51 demonstrated increase during irradiation. further enhancement not due redistribution cell cycle phases. Our results indicate cells triggers different from those elicited after acute activation error-free break pathway suggests compensatory adaptive mechanisms may help alleviate long-term biological consequences could potentially be utilized protection medical practices.