Combination of circulating tumor cell enumeration and tumor marker detection in predicting prognosis and treatment effect in metastatic castration-resistant prostate cancer

• DOI: 10.18632/oncotarget.6167
• Publication Date: 2015-10-23T22:57:02Z
• Authors: Kun Chang; Yun-Yi Kong; Bo Dai; Ding-Wei Ye; Yuan-Yuan Qu; Yue Wang; Zhong-Wei Jia; Gao-Xiang Li
• Keywords: Circulating tumor cell; Enzalutamide

// Kun Chang 1,2,* , Yun-Yi Kong 2,3,* Bo Dai 1,2 Ding-Wei Ye Yuan-Yuan Qu Yue Wang Zhong-Wei Jia and Gao-Xiang Li 1 Department of Urology, Fudan University Shanghai Cancer Center, University, Shanghai, China 2 Oncology, Medical College, 3 Pathology, * Co-first author Correspondence to: Dai, email: Ye, Keywords : circulating tumor cell, epithelial-mesenchymal transition, metastatic castration-resistant prostate cancer, prognosis, stem cell Received August 05, 2015 Accepted October 04, Published 19, Abstract Although (CTC) enumeration in peripheral blood has already been validated as a reliable biomarker predicting prognosis cancer (mCRPC), patients with favorable CTC counts (CTC < 5/7.5 ml) still experience various survival times. Assays that can reduce patients' risks are urgently needed. In this study, we set up real-time quantitative polymerase chain reaction (RT-qPCR) method to detect transition (EMT) gene expression status validate whether they could complement enumeration. From January 2013 June 2014 collected from 70 mCRPC enumerated these samples using CellSearch system. At the same time, cell-related genes (ABCG2, PROM1 PSCA) EMT-related (TWIST1 vimentin) were detected an RT-qPCR assay. Patient overall (OS) treatment methods recorded follow-up. For who received first-line chemotherapy, docetaxel plus prednisone, PSA progression-free (PSA-PFS) response rate recorded. time analysis, 35 had died median follow-up 16.0 months. Unfavorable enumerations ≥5/7.5 predictive shorter OS ( p = 0.01). Also, positive indicated poor However, EMT failed show any prognostic value 0.78). A multivariate analysis serum albumin 0.04), ECOG performance (p 0.01), 0.02) 0.01) independent factors for OS. 40 categorized into group, also suggested combined model consisting increased concordance probability estimated 0.716 0.889 comparison alone. prednisone PSA-PFS low 0.008). did not affect or rates. As result, detection docetaxel-based effects patients.