Tumor invasion and metastasis regulated by microRNA-184 and microRNA-574-5p in small-cell lung cancer

• DOI: 10.18632/oncotarget.6338
• Publication Date: 2015-11-16T18:35:54Z
• Authors: Rui Zhou; Xiaoshu Zhou; Zhongyuan Yin; Jing Guo; Ting Hu; Shun Jiang; Li Liu; Xiaorong Dong; Sheng Zhang; Gang Wu
• Keywords: Male; Lung Neoplasms; Receptor-Like Protein Tyrosine Phosphatases, Class 2; Kaplan-Meier Estimate; Middle Aged; Small Cell Lung Carcinoma; Disease-Free Survival; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; HEK293 Cells; 0302 clinical medicine; Cell Movement; Cell Line, Tumor; Basic Helix-Loop-Helix Transcription Factors; Biomarkers, Tumor; Humans; Female; Neoplasm Invasiveness; 3' Untranslated Regions; Proportional Hazards Models; Signal Transduction

// Rui Zhou 1, * , Xiaoshu Zhongyuan Yin 1 Jing Guo 2 Ting Hu Shun Jiang 3 Li Liu Xiaorong Dong Sheng Zhang Gang Wu Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Department Oncology The Affiliated Hospital Qingdao University, Qingdao, Oncology, Second Xiangya Central South Changsha, These authors have contributed equally to this work Correspondence to: Wu, e-mail: [e-mail] Keywords: miR-184, miR-574-5p, metastasis, prognosis, SCLC Received: April 18, 2015 Accepted: November 06, Published: 16, ABSTRACT Small-cell lung cancer (SCLC) is a highly aggressive neuroendocrine tumor that has an extremely poor clinical prognosis. Metastasis the key event in progression, but its mechanism not been fully elucidated. MicroRNAs (miRNAs) proven participate processes, their function thoroughly studied either. Here, we performed microarray quantitative real-time PCR (qRT-PCR) analysesto identify miRNAsassociated with metastasis prognosis as well correlation between serum tissue. We also explored these miRNAs' promising molecular mechanisms by 3′UTR reporter assay immunoblotting. showed thatmiR-184 significantly attenuated SCLC, whereas miR-574–5p enhanced it. Both miRNAs were found β-catenin signaling suppressing protein tyrosine phosphatase receptor type U (PTPRU)orendothelial PAS domain (EPAS1). Furthermore, was verified independent prognostic risk factor for SCLC. Taken together, our findings providea comprehensive analysis miRNA expression pattern indicate may serve potential therapeutic predictors