Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer

• DOI: 10.18632/oncotarget.6899
• Publication Date: 2016-01-13T10:38:08Z
• Authors: Felix Royo; Patricia Zuñiga-G...; Verónica Torrano; Ana Loizaga; Pilar Sanchez-Mos...; Aitziber Ugalde-O...; Esperanza González; Ana R. Cortazar; Laura Palomo; Sonia Fernández-Ruiz; Isabel Lacasa-Vis...; Maria Berdasco; James D. Sutherland; Rosa Barrio; Amaia Zabala-Letona; Natalia Martín-Ma...; Amaia Arruabarren...; Lorea Valcarcel-J...; Alfredo Caro-Mald...; Jorge Gonzalez-Ta...; Guido Cachi-Fuentes; Manel Esteller; Ana M. Aransay; Miguel Unda; Juan M. Falcón-Pérez; Arkaitz Carracedo
• Keywords: Extracellular Vesicles

// Felix Royo 1, 2, * , Patricia Zuñiga-Garcia Verónica Torrano Ana Loizaga 3, Pilar Sanchez-Mosquera 1 Aitziber Ugalde-Olano 4 Esperanza González R. Cortazar Laura Palomo Sonia Fernández-Ruiz Isabel Lacasa-Viscasillas 3 Maria Berdasco 5 James D. Sutherland Rosa Barrio Amaia Zabala-Letona Natalia Martín-Martín Arruabarrena-Aristorena Lorea Valcarcel-Jimenez Alfredo Caro-Maldonado Jorge Gonzalez-Tampan Guido Cachi-Fuentes Manel Esteller M. Aransay 2 Miguel Unda Juan Falcón-Pérez 6, Ω Arkaitz Carracedo 7, CIC bioGUNE, Bizkaia Technology Park, Biscay, Spain Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (Ciberehd), Department of Urology, Basurto University Hospital, Bilbao, Pathology, Cancer Epigenetics and Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, 6 Ikerbasque, Basque Foundation for Science, Bizkaia, 7 Biochemistry Molecular Department, the Country (UPV/EHU), These authors contributed equally to this work Correspondence to: Carracedo, e-mail: [e-mail] Falcón-Pérez, [e-mail] Keywords: extracellular vesicles, exosomes, prostate cancer, urine biomakers Received: August 18, 2015 Accepted: November 26, Published: January 12, 2016 ABSTRACT Extracellular vesicles (EV) are emerging structures with promising properties intercellular communication. In addition, characterization EV in biofluids is an attractive source non-invasive diagnostic, prognostic predictive biomarkers. Here we show that urinary (uEV) from cancer (PCa) patients exhibit genuine differential physical biological compared benign hyperplasia (BPH). Importantly, transcriptomics uEVs led us define decreased abundance Cadherin type (CDH3) transcript uEV PCa patients. Tissue cell line analysis strongly suggested status CDH3 a distal reflection changes expression cadherin tumor. was negatively regulated at genomic, transcriptional, epigenetic level PCa. Our results reveal could represent tool inform about molecular alterations