Metal distribution and metallothionein induction after cadmium exposure in the terrestrial snail Helix aspersa (Gastropoda, Pulmonata)

• DOI: 10.1897/07-232.1
• Publication Date: 2008-06-05T18:45:49Z
• Authors: Florian Hispard; Dietmar Schuler; Annette de Vaufleury; Renaud Scheifler; Pierre-Marie Badot; Reinhard Dallinger
• Keywords: Metallothionein; Helix pomatia; Hepatopancreas

Abstract The aim of the present work was to study effect Cd 2+ exposure on metallothionein (MT) induction and distribution metals (Cd, Cu, Zn) in terrestrial pulmonate Helix aspersa . In particular, soluble nonsoluble pools accumulated their tissue uncontaminated contaminated edible snails were investigated after a two‐week cytosolic pool midgut gland H. , three metal‐specific putative MT isoforms separated following fractionation protocol with diethylaminoethyl cellulose, size‐exclusion chromatography, ultrafiltration, reversed‐phase high‐performance liquid chromatography (RP‐HPLC). Interestingly, one above seems bind both which may addition mobilize, by some intracellular Cu and, thus, perhaps increase fraction. cDNA its translated amino acid sequence ‐binding isoform from snail characterized attributed obtained RP‐HPLC. this Cd‐MT differed similar sequences described other pulmonates, such as pomatia or Arianta arbustorum only few acids ( n = 4 8, respectively). That identified is inducible also shown, chromatographic evidence aside, specific polymerase chain reaction basis, included noninducible housekeeping gene control.