[Cloning and function analysis of promoter of DcCDPK8 from Dendrobium catenatum].

• DOI: 10.19540/j.cnki.cjcmm.20181106.005
• Publication Date: 2019-01-01
• Authors: Yuan Wang; Yanhui Gao; Yu-Qiu Zhu; Jin-Ping Si
• Keywords: Cloning (programming)

DcCDPK8 involved in abiotic stress such as low temperature and signal transduction of hormones ABA MeJA,but the transcriptional regulation is still unclear. In order to study core promoter region gene Dendrobium catenatum explore its mechanism,the sequence was cloned by PCR from D. catenatum. Promoter function studied fusion 5 'terminal deletion GUS gene. The results showed that has a low-temperature responsive element( LTR) between~(-1) 749 bp and-614 bp,two MeJA elements and-230 bp,and one between-614 bp. Three 5'-end different fragments were constructed fuse eukaryotic expression vectors p BI121 with GUS,which transformed into tobacco leaves. activity under cold treatment DcCDPK8-p1>DcCDPK8-p2>DcCDPK8-p3. exogenous induction DcCDPK8-p1>DcCDPK8-p2>DcCDPK8-p3,and It speculated response ARE) sequences positive regulatory role ABA,the cis-acting element plays negative MeJA.