[Effects of Arsenic Disulfide Combined with Itraconazole on Proli- feration and Apoptosis and Hedgehog Pathway of Diffuse Large B-Cell Lymphoma Cells].

• DOI: 10.19746/j.cnki.issn.1009-2137.2021.05.020
• Publication Date: 2021-10-01
• Authors: Ling Wang; Shi-Quan Ding; Huawei Li; Shenghong Du; Chen Chen; Yuyu Liu; Banban Li; Cong Liu; Jun Jiao; Qingliang Teng
• Keywords: Viability assay; GLI1

OBJECTIVE To investigate the effect of arsenic disulfide (AS2S2) combined with itraconazole on proliferation, apoptosis and hedgehog pathway diffuse large B-cell lymphoma (DLBCL) cells. METHODS The human DLBCL cell OCI-LY3 was treated different concentrations AS2S2 itraconazole. Cell proliferation inhibition detected by CCK-8, rate determined flow cytometry. expression levels BCL-2, BAX, SMO GLi1 were Western blot. RESULTS viability decreased significantly at 24, 48 or 72 h as cultured Along increasing concentration, reduced compared that in control group, results showed statistically significant(r=-0.690，r=-0.639, r=-0.833, r=-0.808, r=-0.578). inhibitory rates cells increased those single drug-treated group after combination AS2S2(P<0.05). protein Glil down-regulated alone(P<0.01). combined-treatment group(P<0.01). CONCLUSION Itraconazole can inhibit a concentration-and time-dependent manner. In addition, show synergistic effects, which may be related Hedgehog signaling pathway.