Evaluation of classical swine fever virus antibody detection assays with an emphasis on the differentiation of infected from vaccinated animals

• DOI: 10.20506/rst.31.3.2173
• Publication Date: 2016-03-18T13:56:51Z
• Authors: S. SCHROEDER; T. VON ROSEN; S. BLOME; W. LOEFFEN; A. HAEGERMAN; F. KOENEN; A. UTTENTHAL
• Keywords: Classical swine fever; Diva; Pestivirus

The aim of this study was to evaluate the general characteristics commercially available enzyme-linked immunosorbent assays (ELISAs) detect antibody against classical swine fever (CSF), as well assess their potential use accompanying marker tests able differentiate infected from vaccinated animals (DIVA). Chekit* CSF-Sero and HerdChek* CSFV Ab, both which antibodies E2 protein virus (CSFV), had highest sensitivity. Both were practicable showed good reproducibility. Comparable sensitivity shown by CSF-Marker, an Erns ELISA. However, test does not allow differentiation between directed ruminant pestiviruses those CSFV. Therefore, it is suitable for with chimeric vaccines tested. PrioCHECK only ELISA in DIVA containing proteins pestiviruses. less sensitive selective than E2-ELISAs cannot be recommended.