SELENYUM SİKLOFOSFAMİD NEDENLİ HEPATOTOKSİSİTEYİ İYİLEŞTİREBİLİR / SELENIUM AMELIORATES CYCLOPHOSPHAMIDE-INDUCED HEPATOTOXICITY

• DOI: 10.20515/otd.73102
• Publication Date: 2016-05-21T07:34:22Z
• Authors: Adnan Ayhancı; Özge Acar; Varol Şahintürk; Sibel Güneş; İlknur Kulcanay Ş...; Ahmet Musmul; Sema Uslu
• Keywords: Siklofosfamid;oksidatif stres;hepatotoksisite;selenyum; 0301 basic medicine; 03 medical and health sciences; Health Care Administration; Sağlık Kurumları Yönetimi; 3. Good health

OZET: Siklofosfamid (CP)’in antitumor etkinligi dozu ile dogru orantilidir. Bununla birlikte, yuksek dozlarda yaygin sitotoksisitelere neden olur. Selenyum (Se) yapisina katildigi glutatyon peroksidaz gibi selenoproteinler yoluyla potansiyel besinsel bir antioksidan olarak biyolojik etkiler gostermektedir. Bu nedenle siklofosfamid nedenli hepatotoksisitede selenyumun olasi koruyucu etkisinin arastirilmasi amaclanmistir. Toplam 42 adet erkek Spraque-Dawley sican 6 gruba (n=7) ayrildi (kontrol, 150 mg/kg CP, 0.5 ve 1 mg/kg Se, CP+0.5 ve CP+1 mg/kg Se gruplari). Se’un karaciger toksisitesinde koruyucu etkisini belirlemek icin serum alanin transaminaz (ALT), aspartat transaminaz (AST), alkalen fosfataz (ALP) ve laktat dehidrogenaz (LDH) seviyeleri belirlendi. Ayrica karaciger dokusu histolojik olarak da incelendi. CP uygulanan grupta serum ALT (%145), AST (%226), ALP (%88), ve LDH (%73) duzeyleri artti. CP ile birlikte Se uygulanan gruplarda ALT, AST, ALP ve LDH duzeyleri azaldi ( p <0.05). Histolojik incelemelerde de CP+Se gruplarinda karaciger doku hasari onemli duzeyde azaldi. Sonuclarimiz selenyumun antioksidan etkilerinin oldugu ve siklofosfamid nedenli oksidatif hasari ve coklu organ toksisitelerini gidermede yararli olabilecegini gostermistir. ANAHTAR KELIMELER: Siklofosfamid, oksidatif stres, hepatotoksisite, selenyum, rat. SELENIUM AMELIORATES CYCLOPHOSPHAMIDE-INDUCED HEPATOTOXICITY ABSTRACT: The antitumoral efficieny of CP is directly proportional to its administrated dose. However, high doses have a tendency to result in generalized cytotoxicity. Selenium (Se) is a potent nutritional antioxidant that carries out biological effects by its incorporation into selenoproteins, such as glutathione peroxidase. Therefore we aimed to investigate the possible protective effect of Se on CP-induced hepatotoxicity. A total of 42 male Spraque-Dawley rats were divided into 6 groups (n=7) (control, 150 mg/kg CP, 0.5 or 1 mg/kg Se and CP+0.5 and CP+1 mg/kg Se groups). In order to determine the protective effects of Se on liver toxicity, the levels of serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined. Also, the liver tissues were analysed histologically. Serum ALT (%145), AST (%226), ALP (%88), and LDH (%73) levels increased in CP administrated rats. In groups where CP and Se were given together ALT, AST, ALP, and LDH levels decreased ( p <0.05). Histological analysis of liver tissue showed that tissue damage was significantly lower in CP+Se groups. Our results show that Se has antioxidant effects and that it may be useful to eliminate CP related oxidative damage. KEYWORDS: Cyclophosphamide, oxidative stress, hepatotoxicity, selenium, rat.