Bovine milk extracellular vesicles prepared by ultracentrifugation contain microbial mRNAs that do not accumulate in human plasma following milk consumption

• DOI: 10.20517/evcna.2024.84
• Publication Date: 2025-05-29T02:09:32Z
• Authors: Peerzada T. Mumtaz; Bijaya Upadhyaya; Jiang Shu; Juan Cui; Janos Zempleni
• Keywords: Bovine milk; Extracellular Vesicles

Aim: Small extracellular vesicles (sEVs) and their RNA cargo are not exclusively derived from endogenous synthesis but can also be absorbed milk gut bacteria. Given the high rate of bacterial fermentation in gastrointestinal tract ruminants, we hypothesized that preparations bovine sEVs (BMEs) contain mRNAs whose bioavailability humans remains unknown. Methods: BMEs were purified chilled antibiotics-treated raw (RM) store-bought skim (SBM) using sequential ultracentrifugation. RM treated with RNase to remove adsorbed BME surface. SBM (SBM+) or (SBM-) RNase. identified by sequencing analysis mapping genome reference. The mRNA was assessed plasma collected before 4 h after consuming one liter cow’s humans. Results: Approximately 50% reads non-bovine RM, SBM+, BM-. Up two-thirds contigs mapped microbial transcriptomes, including bacteria, viruses, fungi. levels 17 Escherichia coli Cutibacterium acnes significantly higher consumption compared consumption, number too low confidently draw conclusion bioavailable Conclusions: prepared ultracentrifugation