Secretome and extracellular vesicle signatures in bone marrow-derived mesenchymal stromal cells after expansion in standard and next-generation media

• DOI: 10.20517/evcna.2024.99
• Publication Date: 2025-04-29T10:12:26Z
• Authors: Giulio Grieco; Simona Piccolo; Enrico Ragni; Laura de Girolamo
• Keywords: Extracellular Vesicles; Extracellular vesicles

Aim: Mesenchymal stem cells (MSCs) are a promising therapeutic strategy for osteoarthritis (OA), largely due to their regenerative potential, which is attributed in part secretome. The secretome includes soluble factors and extracellular vesicles (EVs). Given that MSCs sensitive various culture conditions, this study aims investigate the effects of different media supplemented with either fetal bovine serum (FBS) (F), platelet lysate (P), or serum/xeno-free (S/X) on composition potential from bone marrow-derived (BMSCs). Methods: BMSCs were cultured F, P, S/X media, secretomes collected after starvation. analyzed factors, EVs, miRNAs. Inflammatory responses assessed an vitro OA model using inflamed chondrocytes gene expression was evaluated by qRT-PCR. Results: all conditions exhibited similar molecular fingerprint. Proteomic analysis identified 98 common proteins encompassing growth inflammatory mediators. EVs showed size phenotype, slight difference CD44 derived P-expanded MSCs. Despite high overall similarity, miRNA profiling 13 key players, subtle differences FBS-expanded BMSCs. All anti-inflammatory effects, showing most pronounced potential. Conclusion: share components. may contribute variations outcomes through cargo. Optimizing MSC expansion crucial enhancing MSC-derived treatment. Further research needed clarify specific role miRNAs, modulating pathology.