The Hong Kong genome project: building genome sequencing capacity and capability for advancing genomic science in Hong Kong

• DOI: 10.20517/jtgg.2023.22
• Publication Date: 2023-10-27T08:21:45Z
• Authors: Annie T. W. Chu; Amy H. Y. Tong; Desiree M. S. Tse; Cario W. S. Lo; Carol C. F. Lau; Cecilia Y. F. Li; Nattily S. Y. Tai; Lap W. Wong; Gigi K. C. Choy; Belinda Y. Y. Tse; Su-vui Lo; Ken Sung; Mullin Yu; Hong Kong Genome...; Brian H. Y. Chung
• Keywords: Personal genomics; Sample (material)

Aim: The Hong Kong Genome Project (HKGP) is the first large-scale genome sequencing (GS) project in Special Administrative Region. Institute (HKGI) entrusted with task of implementing HKGP. With aim to sequence 45,000-50,000 genomes five years, it project’s goal provide participants more precise diagnosis and personalised treatment, drive application integration genomic medicine into routine clinical care. Methods: HKGI Laboratory’s hardware software components were customised tailor needs project. Sample handling storage protocol, DNA extraction, PCR-free GS workflow developed optimised. Quality control indicators metrics for assessing quality samples, libraries data established. Results: Laboratory designed facilitate a unidirectional minimise risk contamination. Manager system handles laboratory generated from HKGP samples biobank. analyses approximately 350-500 per week, majority which are whole blood. During 24 months since launch HKGP, 12,937 their family members (6,680 genomes) have been recruited sequenced. capacity has further enhanced include latest technologies, such as long-read multi-omics order meet target Conclusion: established robust utility will bring precision practice.