The Effect of Lyophilized and Frozen Umbilical Cord Cryoextract on L929 Cell Culture

• DOI: 10.20535/ibb.2025.9.1.313718
• Publication Date: 2025-04-14T14:50:00Z
• Authors: Anna Kaverinska; Nadiia Shevchenko; Aleksandr Osetsky; Liudmyla Sukhodub; Viktoriya Lazurenko; Oleksandr Zhelezn...; Volodymyr Prokopiuk

Background. The human umbilical cord is a promising source of biologically active substances with regenerative properties. However, the potential lyophilized cryoextract from for medicine, which could facilitate storage and transportation, remains unexplored. Therefore, it important to study effect such cryoextracts using cellular model. Objective. To evaluate frozen on L929 cell line assess their therapeutic potential. Methods. This was conducted cultures. Cryoextracts were obtained through cryoextraction forms at -80 -20 °C. These extracts added Dulbecco's Modified Eagle Medium (DMEM) three concentrations: 0.1, 0.5, 1.0 mg/ml. control groups included cells cultured in DMEM without fetal bovine serum. Cell morphology monolayer confluency observed. impact cryoextracts, several assays performed: viability (adhesion), migration activity (scratch test), pinocytosis (neutral red uptake assay), metabolic (MTT (proliferation) population doubling time. Results. addition its form °C non-toxic cells. most effective concentration 0.1 mg/ml, significantly stimulated adhesion proliferation compared culture medium did not enhance but increase activity. Conclusions. findings suggest that can be used as growth factors cultivation. shows promise use conditions where specialized equipment available. primarily stimulates inhibits proliferation.