Intestinal Microbial and Metabolite Profiling of Mice Fed with Dietary Glucose and Fructose

• DOI: 10.20944/preprints201801.0210.v1
• Publication Date: 2018-01-26T02:31:40Z
• Authors: João C. P. Silva; Marta Mota; Fátima O. Martins; Célia Nogueira; Teresa Gonçalves; Tatiana Carneiro; Joana Pinto; Daniela Duarte; António S. Barros; John G. Jones; Ana M. Gil
• Keywords: Monosaccharide; Carbohydrate Metabolism

Increased sugar intake is implicated in Type-2 diabetes and fatty liver disease. Mechanisms by which glucose fructose components promote these conditions are unclear. We hypothesize that alterations intestinal metabolite microbiota profiles specific to each monosaccharide involved. Two groups of six adult C57BL/6 mice were fed for 10-weeks with a diet where either or was the sole carbohydrate component (G F, respectively). A third group normal chow (N). Fecal metabolites profiled every 2-weeks 1H NMR microbial composition analysed real-time PCR (qPCR). Glucose tolerance also periodically assessed. N, G F had similar weight gains tolerance. Multivariate analysis indicated separated from both N G, decreased butyrate glutamate increased fructose, succinate, taurine, tyrosine xylose. Compared showed shift microbe populations gram-positive Lactobacillus spp. gram-negative Enterobacteria species. Substitution mixture pure 10 weeks did not alter adiposity However, generated distinctive fecal signatures incomplete absorption as dominant feature mice.