Comprehensive snake venomics of the Okinawa Habu pit viper, Protobothrops flavoviridis, by complementary mass spectrometry-guided approaches

• DOI: 10.20944/preprints201807.0123.v1
• Publication Date: 2018-07-09T23:47:10Z
• Authors: Maik Damm; Benjamin-Florian ...; Ayse Nalbantsoy; Roderich D. Süssmuth
• Keywords: Viper Venoms; Antivenom; Myotoxin; Viperidae

The Asian world is home to a multitude of venomous and dangerous snakes, which are attributed various medical effects used in the preparation traditional snake tinctures alcoholics, like Japanese wine, named Habushu. aim this work was perform first quantitative proteomic analysis Protobothrops flavoviridis pit viper venom. Accordingly, venom analyzed by complimentary bottom-up top-down mass spectrometry techniques. spectrometry-based venomics approach revealed that more than half composed different phospholipases A2 (PLA2). combination with an intact profiling led identification three main Habu PLA2s. Furthermore, nearly one-third total consists metalloproteinases disintegrins, several minor represented toxins families were detected: CTL, CRISP, svSP, LAAO, PDE 5’-nucleotidase. Finally, P. contains certain bradykinin-potentiating peptides related peptides, svMP inhibitors pEKW, pEQW, pEEW pENW. In preliminary MTT cytotoxicity assays highest cancerous-cytotoxicity crude measured against human neuroblastoma SH-SY5Y cells shows some fractions disintegrin-like effects.