Provitamin A Biofortification of Durum Wheat through a TILLING Approach

• DOI: 10.20944/preprints201910.0272.v1
• Publication Date: 2019-10-24T08:19:31Z
• Authors: Francesco Sestili; Maria Dolores Gar...; Gianluca Gambacorta; Romina Beleggia; Ermelinda Botticella; Pasquale De Vita; Daniel-Valentin S...; Stefania Masci; Domenico Lafiandra
• Keywords: Biofortification; Provitamin; TILLING; Micronutrient deficiency

Macro and micronutrients, essential for the maintenance of human metabolism, are daily assimilated through diet. Wheat other major cereals a good source nutrients, such as carbohydrates proteins, but cannot supply enough amounts micronutrients which includes provitamin A. As vitamin A deficiency (VAD) lead to several serious diseases spread worldwide, biofortification staple crop, wheat, represents an effective way preserve health in developing countries. In present work, key enzyme involved branch carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by TILLING approach “Block strategy” perspective. The null mutant genotype showed strong reduction expression lcyE gene also interesting pleiotropic effects on (β-ring hydroxylase) acting downstream pathway. Biochemical profiling wheat lines increase roughly 75% β-carotene grains complete line vs. control. conclusions, here we describe production characterization new biofortified obtained non-transgenic shading light molecular mechanism governing biosynthesis durum wheat.