Development and Validation of A Rapid Lateral Flow E1/E2-Antigen Test and ELISA in Patients Infected with Emerging Asian Strain of Chikungunya Virus in The Americas

0301 basic medicine 610 Enzyme-Linked Immunosorbent Assay Colombia Antibodies, Viral Microbiology Sensitivity and Specificity Article E1/E2 antigen detection 03 medical and health sciences Viral Envelope Proteins alphavirus Humans Serologic Tests Chikungunya fever Antigens, Viral rapid diagnosis Immunoassay 0303 health sciences other QR1-502 3. Good health acute phase diagnosis Latin America lateral flow Honduras Chikungunya Fever ELISA Chikungunya virus
DOI: 10.20944/preprints202008.0309.v1 Publication Date: 2020-08-15T08:22:02Z
ABSTRACT
Since its 2013 emergence in the Americas, chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of disease, though currently lacking clinics, is integral enable timely care epidemiological response. We developed dual detection system: CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) lateral flow test using high-affinity anti-CHIKV antibodies. The ELISA was validated with 100 PCR-tested acute Chikungunya fever samples from Honduras. had an overall sensitivity specificity 51% 96.67%, respectively, accuracy reaching 95.45% 92.03% at Ct cutoff 22. As value increased 22, decreased. then two tests independent antibody pairs. reached 100% for both 39 Colombia Honduras cutoffs 20 27, respectively. For tests, decreased as after 27. Because E1/E2 are exposed virion surfaces serum during infection phase, these sensitive specific assays demonstrate opportunities early this emerging human pathogen.
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