Furin Prodomain Ppfurin Enhances Soce Through trpc6 Activation in Breast Cancer Cells

• DOI: 10.20944/preprints202008.0441.v1
• Publication Date: 2020-08-24T10:47:56Z
• Authors: Jose J. López; Geraldine Siegfried; Carlos Cantonero; Jean Descarpentrie; Tarik Smani; Iker Badiola; Simon Pernot; Serge Evrard; Juan A. Rosado; Abdel-Majid Khatib
• Keywords: Furin; TRPC6

The intracellular calcium concentration ([Ca2+]i) modulation plays a key role in the regulation of cellular growth and survival normal cells failure [Ca2+]i homeostasis is involved tumor initiation progression. Here we show that inhibition Furin by its naturally occurring inhibitor prodomain ppFurin MDA-MB-231 breast cancer resulted enhanced SOCE through TRPC6 activation associated reduced malignant phenotype. Expression stable manner melanoma MDA-MB-435 cell lines inhibits activity as assessed vitro digestion assays. Accordingly, transfection experiments, revealed ppFurin-expressing are unable to process adequately PC substrates proVEGF-C proIGF-1R. Compared cells, expression significantly induces Ca2+ entry which impaired silencing expression. Analysis up-regulated tyrosine phosphorylation cells. their viability ability migrate sensitization apoptosis inducer hydrogen peroxide. These findings suggest may be useful strategy interfere with mobilization leading phenotype repression reduction resistance treatments.