Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific <em>tuf</em> Gene Sequencing

• DOI: 10.20944/preprints202011.0640.v1
• Publication Date: 2020-11-26T09:48:06Z
• Authors: Søren Iversen; Thor Bech Johannesen; Anna Cäcilia Ingham; Sofie Marie Edslev; Staffan Tevell; Emeli Månsson; Åsa Nilsdotter-Au...; Bo Söderquist; Marc Stegger; Paal Skytt Andersen
• Keywords: Groin; Anterior nares

The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty assess the impact chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection samples obtained from elective (n=83) by swabbing anterior nares, skin sites groin site planned surgery, before after analysed 16S rRNA (V3-V4) gene sequencing a complementary targeted tuf approach comprehensively characterise staphylococcal communities. Significant reductions alpha diversity detected for both (p=0.04) (p=0.03) surgery with significant relative Corynebacterium (p=0.001) abundance S. hominis (p=0.01) abundance. In occurred (p=0.02), haemolyticus pasteuri (p=0.003) other staphylococci. aureus colonised 35% nares 26% surgery. epidermidis most abundant species at all sampling sites. No genus increased significantly Application provided auxiliary community profiles allowed species-level characterisation directly low biomass clinical samples.