Characterization of Environmental and Cultivable Antibiotic-Resistant Microbial Communities Associated with Wastewater Treatment

0301 basic medicine 0303 health sciences antibiotic resistance RM1-950 atmospheric_science Article 6. Clean water 3. Good health 12. Responsible consumption culturability wastewater treatment 03 medical and health sciences 11. Sustainability Therapeutics. Pharmacology
DOI: 10.20944/preprints202103.0140.v1 Publication Date: 2021-03-04T07:41:33Z
ABSTRACT
Bacterial resistance to antibiotics is a growing global concern, threatening human and environ-mental health, particularly among urban populations. Wastewater treatment plants (WWTPs) are thought be “hotspots” for antibiotic dissemination. The conditions of WWTPs, in conjunction with the persistence commonly used antibiotics, may favor selection trans-fer genes bacterial WWTPs provide an important ecological niche examine spread resistance. We heterotrophic plate count methods identify phenotypically resistant cultivable portions these communities charac-terized composition culturable subset Resistant taxa were more abundant raw sewage wastewater before biological aeration stage. While some bacteria (ARB) detectable downstream treated re-lease, organisms not enriched relative effluent-free upstream water, indicating effi-cient removal during treatment. Combined culture-dependent culture-independent analyses revealed stark difference community between fractions envi-ronmental source material, irrespective culturing conditions. Higher proportions populations recovered than predicted by widely accepted 1% culturability paradigm. These results represent baseline abundance compositional data ARB commu-nities reference future studies addressing dissemination associ-ated ecosystems.
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