Production of Biomodified Bleached Kraft Pulp by Catalytic Conversion Using Penicillium verruculosum Enzymes: Composition, Properties, Structure and Application

• DOI: 10.20944/preprints202212.0266.v1
• Publication Date: 2022-12-15T08:17:45Z
• Authors: Aleksandr R. Shev...; Irina V. Tyshkunova; Alexey V. Malkov; Evgeniy A. Toptunov; Vadim D. Telitsin; Alexandra M. Rozh...; Olga A. Sinitsyna; Iosif V. Gofman; Andrey S. Aksenov
• Keywords: Enzymatic Hydrolysis

The global development of bioeconomy is impossible without technologies for comprehensive processing plant renewable resources. use proven pretreatment raises the possibility industrial implementation enzymatic conversion polysaccharides from lignocellulose considering process complexity. For instance, a well-tuned kraft pulping produces substrate easily degraded by cellulases and hemicelulases. Enzymatic hydrolysis bleached hardwood pulp was carried out using an enzyme complex endoglucanases, cellobiohydrolases, b-glucosidases xylanases produced recombinant strains Penicillium verruculosum at 10 FPU/g mixture rate % concentration. As result biocatalysis following products are obtained: sugar solution, mainly glucose, xylobiose, xylose, as well other minor reducing sugars; modified based on cellulose xylan. composition biomodified determined involving HPLC. method determining degree crystallinity X-ray diffractometer used to characterize properties. article shows producing cryogels amorphization with concentrated 85 H3PO4 followed precipitation water supercritical drying. Analysis hydrolysate reveals predominance glucose (55...67 %) in sugars maximum content up 6 solution after 72 hours. properties structure were shown change during biocatalysis, particular, increased 5 3 hours hydrolysis. We obtained initial rates 35, 50 70 %. these not inferior those microcrystalline cellulose, confirmed specific surface area, swelling, porosity SEM images. Thus, offers prospects only sugar-rich hydrolysates microbiological synthesis, but also powders specified