Biochemical and Molecular Characterization of Musa sp. Cultured on Temporary Immersion Bioreactor

• DOI: 10.20944/preprints202301.0117.v1
• Publication Date: 2023-01-06T08:49:11Z
• Authors: Christopher A Sam...; Rosalinda Aybar B...; Sullymar Morales ...; Dinorah Andino Sa...; Axel Reyes Colón; Juan A Negron Ber...; Angel Núñez Marrero; Alok Arun
• Keywords: <i>Rubisco</i>; 2. Zero hunger; micropropagation; QK1-989; <i>PEPC</i>; Botany; 15. Life on land; plant_sciences; temporary immersion bioreactor; Article

The genus Musa spp. contains commercially important fleshy fruit producing plants including plantains and bananas with a strong potential of providing food security source revenue to farmers. Occasionally, plantlets are often not available fulfill the supply demand farmers particularly in Caribbean region. Concerns quality vegetative tissues along possibility transmission phytopathogens makes availability limited farm-ers. Micropropagation offers an alternative large number in-vitro plantlets. However, conventional methods micropropagation techniques require high produc-tion costs labor-intensive. Recently, Temporary Immersion Bioreactor (TIB) has emerged as methods. Our work utilized SEM (Scanning Electron Microscope), molecular biochemical tools (qRT-PCR ICP-OES) characterize compare morphological, elemental composition, photosynthetic gene expression cultured on TIB. Additionally, morphological features growth propagation rates were analyzed for comparing outputs obtained from temporary immersion bioreactor (CM) techniques. Results showed higher multiplication cultivated Gene analysis selected genes demonstrated transcript abundance phosphoenol pyruvate carboxylase (PEPC) plantain by Elemental composition content iron grown TIB suggesting correlation PEPC expression. These results demonstrate be efficient method produce healthy plantains.