Identification of a NACC1-Regulated Gene Signature Impli-Cated in the Features of Triple-Negative Breast Cancer

• DOI: 10.20944/preprints202302.0434.v1
• Publication Date: 2023-02-28T02:05:26Z
• Authors: Xia Liu; Chrispus Ngule; Hami Hemati; Oluwafunminiyi Ob...; Amos Oyelami; Felix Oyelami; Xingcong Ren; Jianxun Song; Xiaofang Xiong; Jin-ming Yang
• Keywords: Triple-negative breast cancer; Gene signature; Progesterone receptor; FOXM1

Triple-negative breast cancer (TNBC), characterized by deficiency in estrogen receptor (ER), progesterone (PR), and human epidermal growth factor receptor2 (HER2), is among the most lethal subtypes of (BC). Nevertheless, molecular determinants that contribute to its malignant phenotype such as tumor heterogeneity therapy resistance remain elusive. In this study, we sought identify stemness-associated genes involved TNBC progression. Using bioinformatics approach, found 55-up 9-down regulated TNBC. Out 55 upregulated genes, a 5 gene-signature (CDK1, EZH2, CCNB1, CCNA2, AURKA) cell regeneration, was positively correlated with status hypoxia clustered recognized Parametric Gene Set Enrichment Analysis (PGSEA). Also, enhanced infiltration immunosuppressive cells expression these genes. Moreover, our experiments showed depletion transcriptional co-factor nucleus accumbens-associated protein 1 (NAC1), which highly expressed TNBC, reduced expressions Thus, five signature identified study warrants further exploration potential new biomarker heterogeneity/stemness high hypoxia, stemness enrichment, immune-suppressive microenvironment.