The Dynamics of Gene Expression Unraveling the Immune Response of Macrobrachium rosenbergii Infected by Aeromonas veronii

• DOI: 10.20944/preprints202305.0288.v1
• Publication Date: 2023-05-05T01:49:13Z
• Authors: Xin Peng; Xuan Lan; Zhenxiao Zhong; Haihui Tu; Xinyi Yao; Qiongying Tang; Zhenglong Xia; Guoliang Yang; Shaokui Yi
• Keywords: transcriptome; differentially expressed genes; <i>Macrobrachium rosenbergii</i>; <i>Aeromonas veronii</i>; Glycogen Synthase Kinase 3 beta; Immunity; Animals; Aeromonas veronii; Palaemonidae; Transcriptome; Article; 3. Good health; I-kappa B Kinase

To further investigate the immune response of Macrobrachium rosenbergii against Aeromonas veronii, comparative transcriptomic analyses of the M. rosenbergii hepatopancreas were conducted on challenge and control groups at 6, 12, and 24 h post-infection (hpi), independently. A total of 51,707 high-quality unigenes were collected from the RNA-seq data, and 8,060 differentially expressed genes (DEGs) were discovered through paired comparisons. Among three comparison groups, KEGG pathway enrichment analysis showed that 173 immune-related DEGs were considerably clustered into 28 immune-related pathways, including the lysosome and phagosome, etc. Moreover, the expression levels of the four key immune-related genes (TOLL, PAK1, GSK3β, and IKKα) were evaluated at various stages following post-infection in the hepatopancreas, hemolymph, and gill. Both PAK1 and GSK3β genes were highly upregulated in all three tissues at 6 hpi with A. veronii; TOLL was up-regulated in the hepatopancreas and hemolymph but down-regulated in the gill at 6 hpi; and IKKα was up-regulated in hemolymph and gill, but down-regulated in the hepatopancreas at 6 hpi. These findings lay the groundwork for understanding the immune mechanism of M. rosenbergii after contracting A. veronii.