E7 Oncogene HPV 58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis

• DOI: 10.20944/preprints202306.1166.v1
• Publication Date: 2023-06-19T02:25:25Z
• Authors: Bárbara Simas Chagas; Elias Tibúrcio-Jú...; Ruany Cristyne de...; Daffany Luana dos...; Marconi Rêgo Barr...; Rita de Cássia Pe...; Maria da Conceiçã...; Pedro Luiz França...; Vanessa Emanuelle...; Antonio Hunberto ...; Jacinto Costa Sil...; Marcus Vinícius d...; Antonio Carlos de...
• Keywords: variants; human papillomavirus 58; QH301-705.5; phylogenetic analysis; gene expression; Biology (General); human papillomavirus 58; E7 oncogene; variants; phylogenetic analysis; gene expression; E7 oncogene; Article; 3. Good health

Cervical cancer is associated with persistent infections by high-risk HPV types that may have nucleotide polymorphisms and, consequently, different oncogenic potentials. Therefore, the objective of this study was to evaluate the genetic variability and structural effects of E7 oncogene of HPV58 in cervical scraping samples from Brazilian women. The study was carried out with patients from hospitals in metropolitan area of Recife, PE, Brazil. The most frequent HPV type was HPV16, 18 and 58, respectively. Phylogenetic analysis showed that the isolates were classified as sublineages A2, C1 and D2. Two positively selected mutations were found in E7: 63G and 64T. The mutations G41R, G63D and T64A in E7 protein were predicted to reduce the stability of the protein structure. Regarding the interaction of the E7 variant of HPV58 with the signaling of the NF-kB pathway, we observed that the variant HPV58/UFPE-54S decreased the activity of the pathway when compared to the prototype and the other variants behaved similarly to the prototype and the prototype increased the activity of the pathway when compared to pcDNA. In this study, it was possible to identify mutations that may interfere in the molecular interaction between the viral oncoproteins and host proteins.