Genome-Wide Identification and Expression Analysis of bZIP Family Genes in <em>Stevia rebaudiana</em> Bertoni

• DOI: 10.20944/preprints202307.2017.v1
• Publication Date: 2023-08-01T03:26:26Z
• Authors: Wu Mengyang; Chen Jinsong; Tang Weilin; Jiang Yijie; Hu Zhaoyong; Xu Dongbei; Kai Hou; Chen Yinyin; Wu Wei
• Keywords: Stevia rebaudiana; Protein family

The basic (region) leucine zippers (bZIPs) are evolutionarily conserved transcription factors widely distributed in eukaryotic organisms. In plants, they not only involved growth and development, defense stress responses, regulation of physiological processes, but also play a pivotal role regulating secondary metabolism. To explore the function related to bZIP gene family Stevia rebaudiana Bertoni, we identified 105 SrbZIP genes at genome-wide level classified into 12 subfamilies using bioinformation methods. Analysis physicochemical properties showed that amino acid length readings ranged from 96 aa (SrbZIP13) 704 (SrbZIP17), isoelectric point 4.64 (SrbZIP21) 10.08 (SrbZIP101). majority these proteins (94.29%) were localized nucleus. Three main classes cis-acting elements found promoter regions, including development-related (CAT-box), stress-responsive (LTR/ARE/MBS), phytohormone respon-&#x0D; sive (ABRE/TGACG-mitif/TCA-element). Through protein-protein interac-&#x0D; tion network proteins, mainly four major categories: ABF2/ABF4/ABI5 (SrbZIP51/SrbZIP38/SrbZIP7), phy-&#x0D; tohormone signaling, GBF1/GBF3/GBF4 (SrbZIP29/SrbZIP63/SrbZIP60) environmental AREB3 (SrbZIP88), PAN (SrbZIP12), TGA1 (SrbZIP69), TGA4 (SrbZIP82), TGA7 (SrbZIP31), TGA9 (SrbZIP95), TGA10 (SrbZIP79) HY5 (SrbZIP96) cryptochrome FD (SrbZIP23, SrbZIP24, SrbZIP47, SrbZIP67, SrbZIP68, SrbZIP71, SrbZIP72) promoted flowering. transcriptomic data differentially expressed six S. cultivars (‘023’, ‘110’, ‘1188’, ‘11-14’, ‘GP’ ‘GX’). Moreover, expression levels selected 15 response light, abiotic (low temperature, salt drought), phytohormones (methyl jasmonate, gibberellic salicylic acid) treatment different tissues analyzed utilizing qRT-PCR. Six (SrbZIP54, SrbZIP63, SrbZIP32, SrbZIP45, SrbZIP60 SrbZIP9) further be highly induced by affecting glycoside synthesis. Among them, three SrbZIP63 SrbZIP32) predicted terpenoid synthesis rebaudiana. interaction expanded potential functions genes. This study firstly provided comprehensive report family, laying foundation for research on evolution, regulatory