Cissus antractica -ZnO NPs Induce Apoptosis in A549 Cells through ROS-Mediated p53/Bcl-2/Bax Signaling Pathways and Inhibit Inflammatory Cytokines

• DOI: 10.20944/preprints202308.1217.v2
• Publication Date: 2023-08-23T00:31:31Z
• Authors: Eshrat Jahan Rupa; Jinnatun Nahar; Md. Al-Amin; Jin-Kyu Park; Mohanapriya Murug...; Muhammad Awais; Seung Jin Lee; Il Mun Kim; Li Ling; Deok Chun Yang; Dong Uk Yang; Seok-Kyu Jung
• Keywords: Propidium iodide; MTT assay

Green products have excellent potential for discovering and producing new medicinal products. In recent years, there is a growing interest towards the green synthesis of metal nanoparticles, particularly from plants sources. This eco-friendly approach was used in research to biosynthesize zinc oxide nanoparticles (ZnO NPs) Cissus antractica plant. The present study aims examine cell cytotoxicity, death, pathways apoptosis lung cancer cells (A549) inflammation properties treated with biosynthesized ZnO NPs. Additionally, synthe-sized were characterized using UV–vis spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), Energy-dispersive (EDS). XRD TEM analyses exhibited 31 nm as size synthesized pure crystal form NPs, shape NPs nearly spherical hexagonal quartzite. MTT assay cytotoxicity evaluation de-picted significant toxic effect CA-ZnONPs against A549 at concentra-tions up 20 µg/mL than non-cancerous cells. Furthermore, we observed an elevated ROS level These also demonstrated dose-dependent ability suppress colony for-mation migration, which increases their treatment. Further-more, formulation's induce further supported by Hoechst propidium iodide dye. Further, CA increased gene expression BAX, Cyto-c, Caspase 3 9, but decreased Bcl-2, indicating that nanoformulation triggered mitochondrial-mediated apoptosis. Besides, this showed suppressed in-flammatory responses subsequently decreasing reactive oxygen species (ROS) generation, downregulating pro-inflammatory cytokines TNF-α, iNOS, COX-2, IL-6, IL-8, NO. Moreover, study's findings imply are source can might be investigated progress an-ti-inflammatory anti-cancer drugs study.